human keratinocyte cell line hacat (ATCC)

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ATCC human keratinocyte cell line hacat
Viability of <t>HaCaT</t> after 24 h of exposure at GOQDs concentrations ranging from 8 to 500 mg/mL. Cell viability was expressed as a percentage relative to the control (100%).

Viability of <t>HaCaT</t> after 24 h of exposure at GOQDs concentrations ranging from 8 to 500 mg/mL. Cell viability was expressed as a percentage relative to the control (100%).

Human Keratinocyte Cell Line Hacat, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 860 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+keratinocyte+cell+line+hacat/Primary+Epidermal+Keratinocytes%3B+Normal%2C+Human%2C+Adult/pmc13191492-91-1-8
Average 99 stars, based on 860 article reviews

human keratinocyte cell line hacat - by Bioz Stars, 2026-10

99/100 stars

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1) Product Images from "Nitrogen and Boron Co-Doped Graphene Oxide Quantum Dots: Top-Down Fabrication, Comprehensive Characterization and Antibacterial Activity Against Multidrug-Resistant ESKAPE Pathogens"

Journal: ACS Omega

doi: 10.1021/acsomega.6c01888

Viability of HaCaT after 24 h of exposure at GOQDs concentrations ranging from 8 to 500 mg/mL. Cell viability was expressed as a percentage relative to the control (100%).

Figure Legend Snippet: Viability of HaCaT after 24 h of exposure at GOQDs concentrations ranging from 8 to 500 mg/mL. Cell viability was expressed as a percentage relative to the control (100%).

Techniques Used: Control

Cell Culture:

Article Title: Nitrogen and Boron Co-Doped Graphene Oxide Quantum Dots: Top-Down Fabrication, Comprehensive Characterization and Antibacterial Activity Against Multidrug-Resistant ESKAPE Pathogens
Article Snippet: .. The human keratinocyte cell line HaCat (immortalized keratinocytes; ATCC PCS-200-011) was cultured in Dulbecco’s Modified Eagle’s Medium containing 4.5 g/L glucose, l -glutamine, and sodium pyruvate (DMEM, Corning), supplemented with 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin (PEST). .. Cells were seeded into 96-well plates at a density of 1 × 10 5 cells per well, using a Countess Automated Cell Counter (Invitrogen), and incubated overnight at 37 °C in a humidified atmosphere with 5% CO 2 .

Modification:

Isolation:

Article Title: Tattoo Ink Metal Nanoparticles: Assessment of Toxicity In Vitro and with a Novel Human Ex Vivo Model
Article Snippet: .. The human keratinocyte cell line HaCaT (ATCC ® , PCS-200-011) and primary cultures of human keratinocytes and fibroblasts, previously isolated in our institute from donated biopsies from healthy individuals (Ethical Committee protocol n. 581/3, 2019), were used. .. HaCaT cells and primary human fibroblasts were maintained in culture with DMEM supplemented with 10% FBS, 1% glutamine (Sigma-Aldrich), and 1% antibiotics (10,000 units/mL streptomycin and 10,000 units/mL penicillin) (Sigma-Aldrich) at 37 °C with 5% CO 2 .

Article Title: Tattoo Ink Metal Nanoparticles: Assessment of Toxicity In Vitro and with a Novel Human Ex Vivo Model
Article Snippet: .. The human keratinocyte cell line HaCaT (ATCC®, PCS-200-011) and primary cultures of human keratinocytes and fibroblasts, previously isolated in our institute from donated biopsies from healthy individuals (Ethical Committee protocol n. 581/3, 2019), were used. .. HaCaT cells and primary human fibroblasts were maintained in culture with DMEM supplemented with 10% FBS, 1% glutamine (Sigma-Aldrich), and 1% antibiotics (10,000 units/mL streptomycin and 10,000 units/mL penicillin) (Sigma-Aldrich) at 37 ◦C with 5% CO2.

In Vitro:

Article Title: Regenerative and anti-inflammatory effect of a novel bentonite complex on burn wounds
Article Snippet: .. In vitro assay The porcine lung macrophage cell line 3D4/2 (ATCC CRL-2845) and the human keratinocyte cell line HacaT (ATCC PCS-200-011) were used for the in vitro assays. .. The cells were grown at 37°C and 5% CO2 in culture media containing 10% foetal bovine serum (Gibco, Waltham, MA, USA) and 1% penicillinstreptomycin (10,000 unit/mL; Gibco).

Article Title: Regenerative and anti‐inflammatory effect of a novel bentonite complex on burn wounds
Article Snippet: .. The porcine lung macrophage cell line 3D4/2 (ATCC CRL‐2845) and the human keratinocyte cell line HacaT (ATCC PCS‐200‐011) were used for the in vitro assays. .. The cells were grown at 37°C and 5% CO 2 in culture media containing 10% foetal bovine serum (Gibco, Waltham, MA, USA) and 1% penicillin‐streptomycin (10,000 unit/ml; Gibco).

Variant Assay:

Article Title: Methods and compositions for enhancing vaccine immune responses
Article Snippet: .. More specifically, MVA-BN or a derivative or variant of MVA-BN has preferably also the capability of reproductive replication in chicken embryo fibroblasts (CEF), but no capability of reproductive replication in the human keratinocyte cell line HaCat [Boukamp et al (1988), J Cell Biol 106: 761-771], the human bone osteosarcoma cell line 143B (ECACC No. 91112502), the human embryo kidney cell line 293 (ECACC No. 85120602), and the human cervix adenocarcinoma cell line HeLa (ATCC No. CCL-2). ..

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